5O5I

Robo1 Ig5

Summary for 5O5I

• Entry DOI: 10.2210/pdb5o5i/pdb
• Descriptor: Roundabout homolog 1 (2 entities in total)
• Functional Keywords: neuronal receptor, signaling protein
• Biological source: Homo sapiens (Human)
• Cellular location: Cell membrane ; Single-pass type I membrane protein : Q9Y6N7
• Total number of polymer chains: 1
• Total formula weight: 9813.10

Authors

Aleksandrova, N.,Gutsche, I.,Kandiah, E.,Avilov, S.V.,Petoukhov, M.V.,Seiradake, E.,McCarthy, A.A. (deposition date: 2017-06-01, release date: 2018-01-17, Last modification date: 2024-01-17)

Primary citation

Aleksandrova, N.,Gutsche, I.,Kandiah, E.,Avilov, S.V.,Petoukhov, M.V.,Seiradake, E.,McCarthy, A.A.
Robo1 Forms a Compact Dimer-of-Dimers Assembly.
Structure, 26:320-328.e4, 2018

PubMed Abstract: Roundabout (Robo) receptors provide an essential repulsive cue in neuronal development following Slit ligand binding. This important signaling pathway can also be hijacked in numerous cancers, making Slit-Robo an attractive therapeutic target. However, little is known about how Slit binding mediates Robo activation. Here we present the crystal structure of Robo1 Ig1-4 and Robo1 Ig5, together with a negative stain electron microscopy reconstruction of the Robo1 ectodomain. These results show how the Robo1 ectodomain is arranged as compact dimers, mainly mediated by the central Ig domains, which can further interact in a "back-to-back" fashion to generate a tetrameric assembly. We also observed no change in Robo1 oligomerization upon interaction with the dimeric Slit2-N ligand using fluorescent imaging. Taken together with previous studies we propose that Slit2-N binding results in a conformational change of Robo1 to trigger cell signaling.

PubMed: 29307485
DOI: 10.1016/j.str.2017.12.003

Experimental method

X-RAY DIFFRACTION (3.01 Å)