5O0Q

Deglycosylated Nogo Receptor with native disulfide structure

Summary for 5O0Q

• Entry DOI: 10.2210/pdb5o0q/pdb
• Descriptor: Reticulon-4 receptor, 2-acetamido-2-deoxy-beta-D-glucopyranose, PHOSPHATE ION, ... (6 entities in total)
• Functional Keywords: nervous system, signaling, leucine-rich repeat domain, disulfide structure, signaling protein
• Biological source: Mus musculus (Mouse)
• Cellular location: Cell membrane ; Lipid-anchor, GPI-anchor : Q99PI8
• Total number of polymer chains: 4
• Total formula weight: 152227.25

Authors

Pronker, M.F.,Janssen, B.J.C. (deposition date: 2017-05-16, release date: 2017-10-18, Last modification date: 2024-10-16)

Primary citation

Pronker, M.F.,Tas, R.P.,Vlieg, H.C.,Janssen, B.J.C.
Nogo Receptor crystal structures with a native disulfide pattern suggest a novel mode of self-interaction.
Acta Crystallogr D Struct Biol, 73:860-876, 2017

PubMed Abstract: The Nogo Receptor (NgR) is a glycophosphatidylinositol-anchored cell-surface protein and is a receptor for three myelin-associated inhibitors of regeneration: myelin-associated glycoprotein, Nogo66 and oligodendrocyte myelin glycoprotein. In combination with different co-receptors, NgR mediates signalling that reduces neuronal plasticity. The available structures of the NgR ligand-binding leucine-rich repeat (LRR) domain have an artificial disulfide pattern owing to truncated C-terminal construct boundaries. NgR has previously been shown to self-associate via its LRR domain, but the structural basis of this interaction remains elusive. Here, crystal structures of the NgR LRR with a longer C-terminal segment and a native disulfide pattern are presented. An additional C-terminal loop proximal to the C-terminal LRR cap is stabilized by two newly formed disulfide bonds, but is otherwise mostly unstructured in the absence of any stabilizing interactions. NgR crystallized in six unique crystal forms, three of which share a crystal-packing interface. NgR crystal-packing interfaces from all eight unique crystal forms are compared in order to explore how NgR could self-interact on the neuronal plasma membrane.

PubMed: 29095159
DOI: 10.1107/S2059798317013791

Experimental method

X-RAY DIFFRACTION (2.5 Å)