5NUV

Structure of the WD40-domain of human ATG16L1

Summary for 5NUV

• Entry DOI: 10.2210/pdb5nuv/pdb
• Descriptor: Autophagy-related protein 16-1, (R,R)-2,3-BUTANEDIOL (3 entities in total)
• Functional Keywords: atg16l1, wd40, seven-bladed beta-propeller, protein binding
• Biological source: Homo sapiens (Human)
• Cellular location: Cytoplasm : Q676U5
• Total number of polymer chains: 1
• Total formula weight: 33579.17

Authors

Bajagic, M.,Scrima, A. (deposition date: 2017-05-02, release date: 2017-07-19, Last modification date: 2024-01-17)

Primary citation

Bajagic, M.,Archna, A.,Busing, P.,Scrima, A.
Structure of the WD40-domain of human ATG16L1.
Protein Sci., 26:1828-1837, 2017

PubMed Abstract: Autophagy-related protein ATG16L1 is a component of the mammalian ATG12∼ATG5/ATG16L1 complex, which acts as E3-ligase to catalyze lipidation of LC3 during autophagosome biogenesis. The N-terminal part of ATG16L1 comprises the ATG5-binding site and coiled-coil dimerization domain, both also present in yeast ATG16 and essential for bulk and starvation induced autophagy. While absent in yeast ATG16, mammalian ATG16L1 further contains a predicted C-terminal WD40-domain, which has been shown to be involved in mediating interaction with diverse factors in the context of alternative functions of autophagy, such as inflammatory control and xenophagy. In this work, we provide detailed information on the domain boundaries of the WD40-domain of human ATG16L1 and present its crystal structure at a resolution of 1.55 Å.

PubMed: 28685931
DOI: 10.1002/pro.3222

Experimental method

X-RAY DIFFRACTION (1.55 Å)