5NSS

Cryo-EM structure of RNA polymerase-sigma54 holoenzyme with promoter DNA and transcription activator PspF intermedate complex

Summary for 5NSS

Entry DOI	10.2210/pdb5nss/pdb
EMDB information	3696
Descriptor	DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta',DNA-directed RNA polymerase subunit beta',DNA-directed RNA polymerase subunit beta', ... (8 entities in total)
Functional Keywords	transcription initiation, transcription intermediate complex, rna polymerase, sigma54, transcription
Biological source	Escherichia coli K-12 More
Total number of polymer chains	14
Total formula weight	693938.92
Authors	Glyde, R.,Ye, F.Z.,Darbari, V.C.,Zhang, N.,Buck, M.,Zhang, X.D. (deposition date: 2017-04-26, release date: 2017-06-28, Last modification date: 2019-12-11)
Primary citation	Glyde, R.,Ye, F.,Darbari, V.C.,Zhang, N.,Buck, M.,Zhang, X. Structures of RNA Polymerase Closed and Intermediate Complexes Reveal Mechanisms of DNA Opening and Transcription Initiation. Mol. Cell, 67:106-116.e4, 2017 Cited by PubMed: 28579332 DOI: 10.1016/j.molcel.2017.05.010 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (5.8 Å)

Structure validation