5NSR

Cryo-EM structure of RNA polymerase-sigma54 holo enzyme with promoter DNA closed complex

Summary for 5NSR

• Entry DOI: 10.2210/pdb5nsr/pdb
• EMDB information: 3695
• Descriptor: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (7 entities in total)
• Functional Keywords: transcription initiation, rna polymerase, sigma54, transcription
• Biological source: Escherichia coli K-12; More
• Total number of polymer chains: 8
• Total formula weight: 490557.16

Authors

Glyde, R.,Ye, F.Z.,Darbari, V.C.,Zhang, N.,Buck, M.,Zhang, X.D. (deposition date: 2017-04-26, release date: 2017-06-28, Last modification date: 2019-12-11)

• Primary citation: Glyde, R.,Ye, F.,Darbari, V.C.,Zhang, N.,Buck, M.,Zhang, X.; Structures of RNA Polymerase Closed and Intermediate Complexes Reveal Mechanisms of DNA Opening and Transcription Initiation.; Mol. Cell, 67:106-116.e4, 2017; PubMed: 28579332; DOI: 10.1016/j.molcel.2017.05.010

Experimental method

ELECTRON MICROSCOPY (3.8 Å)