5NPY

Crystal structure of Helicobacter pylori flagellar hook protein FlgE2

5NPY の概要

• エントリーDOI: 10.2210/pdb5npy/pdb
• 分子名称: Flagellar basal body protein, TRIS-HYDROXYMETHYL-METHYL-AMMONIUM (3 entities in total)
• 機能のキーワード: helicobactyer pylori, flagellum, hook, motor protein
• 由来する生物種: Helicobacter pylori
• 細胞内の位置: Bacterial flagellum basal body : B5Z7R4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 69027.11

構造登録者

Loconte, V.,Zanotti, G.,Kekez, I.,Matkovic-Calogovic, D. (登録日: 2017-04-19, 公開日: 2017-11-15, 最終更新日: 2024-05-08)

主引用文献

Loconte, V.,Kekez, I.,Matkovic-Calogovic, D.,Zanotti, G.
Structural characterization of FlgE2 protein from Helicobacter pylori hook.
FEBS J., 284:4328-4342, 2017

PubMed Abstract: The Helicobacter pylori flagellum is a complex rotatory nanomachine fundamental for the bacterium's survival in the human stomach. Protein FlgE is a component of the hook, a flexible junction exposed on the cell surface. In the H. pylori genome two different genes are present in different positions coding for hypothetical FlgE. The first protein, FlgE1, is the actual component of the flagellum hook, whilst the second, FlgE2, shares only 26% of the sequence identity with the other and its physiological function is still undefined. We have cloned, purified and crystallized FlgE2, whose structure, determined by the single-wavelength anomalous diffraction method, shows that in overall organization, the protein is composed of three distinct domains, two of them relatively similar to those of FlgE from other Gram-negative bacteria, whilst the third is peculiar to H. pylori. The crystal structure, along with the detected interaction with the regulatory cap protein FlgD, suggests a complementary function of FlgE1 and FlgE2 in the H. pylori flagellum, possibly typical of polar flagella, confirming the role of both proteins in the flagellar hook organization. Although some general features are shared with other Gram-negative bacteria, the presence of two different hook proteins indicates that the molecular organization of H. pylori flagellum has its own peculiarities.

PubMed: 29083539
DOI: 10.1111/febs.14312

実験手法

X-RAY DIFFRACTION (2.292 Å)