5NO2

RsgA-GDPNP bound to the 30S ribosomal subunit (RsgA assembly intermediate)

5NO2 の概要

• エントリーDOI: 10.2210/pdb5no2/pdb
• EMDBエントリー: 3661
• 分子名称: 16S ribosomal RNA, 30S ribosomal protein S12, 30S ribosomal protein S13, ... (22 entities in total)
• 機能のキーワード: ribosome
• 由来する生物種: Escherichia coli (strain K12); 詳細
• 細胞内の位置: Cytoplasm : P39286
• タンパク質・核酸の鎖数: 19
• 化学式量合計: 733041.84

構造登録者

Lopez-Alonso, J.P.,Kaminishi, T.,Kikuchi, T.,Hirata, Y.,Iturrioz, I.,Dhimole, N.,Schedlbauer, A.,Hase, Y.,Goto, S.,Kurita, D.,Muto, A.,Zhou, S.,Naoe, C.,Mills, D.J.,Gil-Carton, D.,Takemoto, C.,Himeno, H.,Fucini, P.,Connell, S.R. (登録日: 2017-04-10, 公開日: 2017-05-24, 最終更新日: 2024-04-24)

主引用文献

Lopez-Alonso, J.P.,Kaminishi, T.,Kikuchi, T.,Hirata, Y.,Iturrioz, I.,Dhimole, N.,Schedlbauer, A.,Hase, Y.,Goto, S.,Kurita, D.,Muto, A.,Zhou, S.,Naoe, C.,Mills, D.J.,Gil-Carton, D.,Takemoto, C.,Himeno, H.,Fucini, P.,Connell, S.R.
RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket.
Nucleic Acids Res., 45:6945-6959, 2017

PubMed Abstract: During 30S ribosomal subunit biogenesis, assembly factors are believed to prevent accumulation of misfolded intermediate states of low free energy that slowly convert into mature 30S subunits, namely, kinetically trapped particles. Among the assembly factors, the circularly permuted GTPase, RsgA, plays a crucial role in the maturation of the 30S decoding center. Here, directed hydroxyl radical probing and single particle cryo-EM are employed to elucidate RsgA΄s mechanism of action. Our results show that RsgA destabilizes the 30S structure, including late binding r-proteins, providing a structural basis for avoiding kinetically trapped assembly intermediates. Moreover, RsgA exploits its distinct GTPase pocket and specific interactions with the 30S to coordinate GTPase activation with the maturation state of the 30S subunit. This coordination validates the architecture of the decoding center and facilitates the timely release of RsgA to control the progression of 30S biogenesis.

PubMed: 28482099
DOI: 10.1093/nar/gkx324

実験手法

ELECTRON MICROSCOPY (5.16 Å)