5NLG

RRP5 C-terminal domain

5NLG の概要

• エントリーDOI: 10.2210/pdb5nlg/pdb
• 分子名称: rRNA biogenesis protein RRP5 (2 entities in total)
• 機能のキーワード: ribosome biogenesis 90s tpr domain, nuclear protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36701.41

構造登録者

Thore, S.,Fribourg, S. (登録日: 2017-04-04, 公開日: 2018-08-29, 最終更新日: 2024-05-08)

主引用文献

Perebaskine, N.,Thore, S.,Fribourg, S.
Structural and interaction analysis of the Rrp5 C-terminal region.
FEBS Open Bio, 8:1605-1614, 2018

PubMed Abstract: Rrp5 is an essential factor during the ribosome biogenesis process. The protein contains a series of 12 S1 RNA-binding domains followed by a TetratricoPeptide Repeat (TPR) domain. In the past, several studies aiming at defining the function of the TPR domain have used nonequivalent Rrp5 constructs, as these protein fragments include not only the TPR module, but also three or four S1 domains. We solved the structure of the Rrp5 TPR module and demonstrated that the TPR region alone does not bind RNA, while the three S1 domains preceding the TPR module can associate with homopolymeric RNA. Finally, we tested the association of our Rrp5 constructs with several proposed interactors, in support of cryo-EM-based models.

PubMed: 30338212
DOI: 10.1002/2211-5463.12495

実験手法

X-RAY DIFFRACTION (2.35 Å)