5NIL

Structure of the MacAB-TolC ABC-type tripartite multidrug efflux pump-MacB section

5NIL の概要

• エントリーDOI: 10.2210/pdb5nil/pdb
• EMDBエントリー: 3653
• 分子名称: Outer membrane protein TolC, Macrolide export protein MacA, Macrolide export ATP-binding/permease protein MacB (3 entities in total)
• 機能のキーワード: abc transporter, drug efflux pump, multi-drug resistance, macrolide transporter, toxin transporter, transport protein
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 545014.30

構造登録者

Fitzpatrick, A.W.P.,Llabres, S.,Neuberger, A.,Blaza, J.N.,Bai, X.-C.,Okada, U.,Murakami, S.,van Veen, H.W.,Zachariae, U.,Scheres, S.H.W.,Luisi, B.F.,Du, D. (登録日: 2017-03-24, 公開日: 2017-05-24, 最終更新日: 2024-05-15)

主引用文献

Fitzpatrick, A.W.P.,Llabres, S.,Neuberger, A.,Blaza, J.N.,Bai, X.C.,Okada, U.,Murakami, S.,van Veen, H.W.,Zachariae, U.,Scheres, S.H.W.,Luisi, B.F.,Du, D.
Structure of the MacAB-TolC ABC-type tripartite multidrug efflux pump.
Nat Microbiol, 2:17070-17070, 2017

PubMed Abstract: The MacA-MacB-TolC assembly of Escherichia coli is a transmembrane machine that spans the cell envelope and actively extrudes substrates, including macrolide antibiotics and polypeptide virulence factors. These transport processes are energized by the ATPase MacB, a member of the ATP-binding cassette (ABC) superfamily. We present an electron cryo-microscopy structure of the ABC-type tripartite assembly at near-atomic resolution. A hexamer of the periplasmic protein MacA bridges between a TolC trimer in the outer membrane and a MacB dimer in the inner membrane, generating a quaternary structure with a central channel for substrate translocation. A gating ring found in MacA is proposed to act as a one-way valve in substrate transport. The MacB structure features an atypical transmembrane domain with a closely packed dimer interface and a periplasmic opening that is the likely portal for substrate entry from the periplasm, with subsequent displacement through an allosteric transport mechanism.

PubMed: 28504659
DOI: 10.1038/nmicrobiol.2017.70

実験手法

ELECTRON MICROSCOPY (5.3 Å)