5NF8

Solution structure of detergent-solubilized Rcf1, a yeast mitochondrial inner membrane protein involved in respiratory Complex III/IV supercomplex formation

5NF8 の概要

• エントリーDOI: 10.2210/pdb5nf8/pdb
• NMR情報: BMRB: 34115
• 分子名称: Respiratory supercomplex factor 1, mitochondrial (1 entity in total)
• 機能のキーワード: homodimer, solution structure, charged dimer interface, membrane protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 40776.99

構造登録者

Zhou, S.,Pettersson, P.,Sjoholm, J.,Sjostrand, D.,Hogbom, M.,Brzezinski, P.,Maler, L.,Adelroth, P. (登録日: 2017-03-13, 公開日: 2018-02-28, 最終更新日: 2024-06-19)

主引用文献

Zhou, S.,Pettersson, P.,Huang, J.,Sjoholm, J.,Sjostrand, D.,Pomes, R.,Hogbom, M.,Brzezinski, P.,Maler, L.,Adelroth, P.
Solution NMR structure of yeast Rcf1, a protein involved in respiratory supercomplex formation.
Proc. Natl. Acad. Sci. U.S.A., 115:3048-3053, 2018

PubMed Abstract: The respiratory supercomplex factor 1 (Rcf1) protein is located in the mitochondrial inner membrane where it is involved in formation of supercomplexes composed of respiratory complexes III and IV. We report the solution structure of Rcf1, which forms a dimer in dodecylphosphocholine (DPC) micelles, where each monomer consists of a bundle of five transmembrane (TM) helices and a short flexible soluble helix (SH). Three TM helices are unusually charged and provide the dimerization interface consisting of 10 putative salt bridges, defining a "charge zipper" motif. The dimer structure is supported by molecular dynamics (MD) simulations in DPC, although the simulations show a more dynamic dimer interface than the NMR data. Furthermore, CD and NMR data indicate that Rcf1 undergoes a structural change when reconstituted in liposomes, which is supported by MD data, suggesting that the dimer structure is unstable in a planar membrane environment. Collectively, these data indicate a dynamic monomer-dimer equilibrium. Furthermore, the Rcf1 dimer interacts with cytochrome , suggesting a role as an electron-transfer bridge between complexes III and IV. The Rcf1 structure will help in understanding its functional roles at a molecular level.

PubMed: 29507228
DOI: 10.1073/pnas.1712061115

実験手法

SOLUTION NMR