5NE5

Crystal structure of family 47 alpha-1,2-mannosidase from Caulobacter K31 strain in complex with kifunensine

5NE5 の概要

• エントリーDOI: 10.2210/pdb5ne5/pdb
• 分子名称: Mannosyl-oligosaccharide 1,2-alpha-mannosidase, KIFUNENSINE, CALCIUM ION, ... (6 entities in total)
• 機能のキーワード: mannosidase glycosidase hydrolysis inhibitor, hydrolase
• 由来する生物種: Caulobacter sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 51091.25

構造登録者

Males, A.,Davies, G.J. (登録日: 2017-03-09, 公開日: 2017-03-29, 最終更新日: 2024-05-08)

主引用文献

Males, A.,Raich, L.,Williams, S.J.,Rovira, C.,Davies, G.J.
Conformational Analysis of the Mannosidase Inhibitor Kifunensine: A Quantum Mechanical and Structural Approach.
Chembiochem, 18:1496-1501, 2017

PubMed Abstract: The varied yet family-specific conformational pathways used by individual glycoside hydrolases (GHs) offer a tantalising prospect for the design of tightly binding and specific enzyme inhibitors. A cardinal example of a GH-family-specific inhibitor, and one that finds widespread practical use, is the natural product kifunensine, which is a low-nanomolar inhibitor that is selective for GH family 47 inverting α-mannosidases. Here we show, through quantum-mechanical approaches, that kifunensine is restrained to a "ring-flipped" C conformation with another accessible, but higher-energy, region around the B conformation. The conformations of kifunensine in complex with a range of GH47 enzymes-including an atomic-level resolution (1 Å) structure of kifunensine with Caulobacter sp. CkGH47 reported herein and with GH family 38 and 92 α-mannosidases-were mapped onto the kifunensine free-energy landscape. These studies revealed that kifunensine has the ability to mimic the product state of GH47 enzymes but cannot mimic any conformational states relevant to the reaction coordinate of mannosidases from other families.

PubMed: 28493500
DOI: 10.1002/cbic.201700166

実験手法

X-RAY DIFFRACTION (1.05 Å)