5NBC

Structure of Prokaryotic Transcription Factors

Summary for 5NBC

• Entry DOI: 10.2210/pdb5nbc/pdb
• Descriptor: Ferric uptake regulation protein, MANGANESE (II) ION, ZINC ION, ... (4 entities in total)
• Functional Keywords: virulence factor, dna binding protein
• Biological source: Francisella tularensis
• Total number of polymer chains: 4
• Total formula weight: 65159.91

Authors

Perard, J.,Carpentier, P.,Michaud-Soret, I.,Cavazza, C. (deposition date: 2017-03-01, release date: 2018-05-16, Last modification date: 2024-05-08)

Primary citation

Perard, J.,Nader, S.,Levert, M.,Arnaud, L.,Carpentier, P.,Siebert, C.,Blanquet, F.,Cavazza, C.,Renesto, P.,Schneider, D.,Maurin, M.,Coves, J.,Crouzy, S.,Michaud-Soret, I.
Structural and functional studies of the metalloregulator Fur identify a promoter-binding mechanism and its role inFrancisella tularensisvirulence.
Commun Biol, 1:93-93, 2018

PubMed Abstract: is a Gram-negative bacterium causing tularaemia. Classified as possible bioterrorism agent, it may be transmitted to humans via animal infection or inhalation leading to severe pneumonia. Its virulence is related to iron homeostasis involving siderophore biosynthesis directly controlled at the transcription level by the ferric uptake regulator Fur, as presented here together with the first crystal structure of the tetrameric Fur in the presence of its physiological cofactor, Fe. Through structural, biophysical, biochemical and modelling studies, we show that promoter sequences of containing Fur boxes enable this tetrameric protein to bind them by splitting it into two dimers. Furthermore, the critical role of Fur in virulence and pathogenesis is demonstrated with a -deleted mutant showing an attenuated virulence in macrophage-like cells and mice. Together, our study suggests that Fur is an attractive target of new antibiotics that attenuate the virulence of .

PubMed: 30271974
DOI: 10.1038/s42003-018-0095-6

Experimental method

X-RAY DIFFRACTION (1.699 Å)