5N9Q

Structure of A. thaliana RCD1(468-567)

5N9Q の概要

• エントリーDOI: 10.2210/pdb5n9q/pdb
• NMR情報: BMRB: 27034
• 分子名称: Inactive poly [ADP-ribose] polymerase RCD1 (1 entity in total)
• 機能のキーワード: helical bundle, transferase
• 由来する生物種: Arabidopsis thaliana (Thale cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13935.75

構造登録者

Tossavainen, H.,Hellman, M.,Vainonen, J.,Kangasjarvi, J.,Permi, P. (登録日: 2017-02-27, 公開日: 2018-03-14, 最終更新日: 2024-06-19)

主引用文献

Shapiguzov, A.,Vainonen, J.P.,Hunter, K.,Tossavainen, H.,Tiwari, A.,Jarvi, S.,Hellman, M.,Aarabi, F.,Alseekh, S.,Wybouw, B.,Van Der Kelen, K.,Nikkanen, L.,Krasensky-Wrzaczek, J.,Sipari, N.,Keinanen, M.,Tyystjarvi, E.,Rintamaki, E.,De Rybel, B.,Salojarvi, J.,Van Breusegem, F.,Fernie, A.R.,Brosche, M.,Permi, P.,Aro, E.M.,Wrzaczek, M.,Kangasjarvi, J.
Arabidopsis RCD1 coordinates chloroplast and mitochondrial functions through interaction with ANAC transcription factors.
Elife, 8:-, 2019

PubMed Abstract: Reactive oxygen species (ROS)-dependent signaling pathways from chloroplasts and mitochondria merge at the nuclear protein RADICAL-INDUCED CELL DEATH1 (RCD1). RCD1 interacts in vivo and suppresses the activity of the transcription factors ANAC013 and ANAC017, which mediate a ROS-related retrograde signal originating from mitochondrial complex III. Inactivation of leads to increased expression of mitochondrial dysfunction stimulon (MDS) genes regulated by ANAC013 and ANAC017. Accumulating MDS gene products, including alternative oxidases (AOXs), affect redox status of the chloroplasts, leading to changes in chloroplast ROS processing and increased protection of photosynthetic apparatus. ROS alter the abundance, thiol redox state and oligomerization of the RCD1 protein in vivo, providing feedback control on its function. RCD1-dependent regulation is linked to chloroplast signaling by 3'-phosphoadenosine 5'-phosphate (PAP). Thus, RCD1 integrates organellar signaling from chloroplasts and mitochondria to establish transcriptional control over the metabolic processes in both organelles.

PubMed: 30767893
DOI: 10.7554/eLife.43284

実験手法

SOLUTION NMR