5N7J

Crystal structure of Neisseria polysaccharea amylosucrase mutant efficient for the synthesis of controlled size maltooligosaccharides

5N7J の概要

• エントリーDOI: 10.2210/pdb5n7j/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900003 PRD_900009
• 分子名称: Amylosucrase, beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: amylosucrase, glycoside hydrolase, glucosyl transferase, mutant, neisseria polysaccharea, gh13, hydrolase
• 由来する生物種: Neisseria polysaccharea
• 細胞内の位置: Secreted : Q9ZEU2
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 73818.25

構造登録者

Verges, A.,Tranier, S. (登録日: 2017-02-20, 公開日: 2017-06-28, 最終更新日: 2024-01-17)

主引用文献

Verges, A.,Barbe, S.,Cambon, E.,Moulis, C.,Tranier, S.,Remaud-Simeon, M.,Andre, I.
Engineering of anp efficient mutant of Neisseria polysaccharea amylosucrase for the synthesis of controlled size maltooligosaccharides.
Carbohydr Polym, 173:403-411, 2017

PubMed Abstract: Amylosucrase from Neisseria polysaccharea naturally catalyzes the synthesis of α-1,4 glucans from sucrose. The product profile is quite polydisperse, ranging from soluble chains called maltooligosaccharides to high-molecular weight insoluble amylose. This enzyme was recently subjected to engineering of its active site to enable recognition of non-natural acceptor substrates. Libraries of variants were constructed and screened on sucrose, allowing the identification of a mutant that showed a 6-fold enhanced activity toward sucrose compared to the wild-type enzyme. Furthermore, its product profile was unprecedented, as only soluble maltooligosaccharides of controlled size chains (2PubMed: 28732882
DOI: 10.1016/j.carbpol.2017.06.011

実験手法

X-RAY DIFFRACTION (2.001 Å)