5N74

Microtubule end binding protein complex

5N74 の概要

• エントリーDOI: 10.2210/pdb5n74/pdb
• 分子名称: Microtubule-associated protein RP/EB family member 1, Karyogamy protein KAR9 (3 entities in total)
• 機能のキーワード: microtubule end binding protein complex, microtubule binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 73699.69

構造登録者

Kumar, A.,Steinmetz, M. (登録日: 2017-02-18, 公開日: 2017-06-14, 最終更新日: 2024-01-17)

主引用文献

Kumar, A.,Manatschal, C.,Rai, A.,Grigoriev, I.,Degen, M.S.,Jaussi, R.,Kretzschmar, I.,Prota, A.E.,Volkmer, R.,Kammerer, R.A.,Akhmanova, A.,Steinmetz, M.O.
Short Linear Sequence Motif LxxPTPh Targets Diverse Proteins to Growing Microtubule Ends.
Structure, 25:924-932.e4, 2017

PubMed Abstract: Microtubule plus-end tracking proteins (+TIPs) are involved in virtually all microtubule-based processes. End-binding (EB) proteins are considered master regulators of +TIP interaction networks, since they autonomously track growing microtubule ends and recruit a plethora of proteins to this location. Two major EB-interacting elements have been described: CAP-Gly domains and linear SxIP sequence motifs. Here, we identified LxxPTPh as a third EB-binding motif that enables major +TIPs to interact with EBs at microtubule ends. In contrast to EB-SxIP and EB-CAP-Gly, the EB-LxxPTPh binding mode does not depend on the C-terminal tail region of EB. Our study reveals that +TIPs developed additional strategies besides CAP-Gly and SxIP to target EBs at growing microtubule ends. They further provide a unique basis to discover novel +TIPs, and to dissect the role of key interaction nodes and their differential regulation for hierarchical +TIP network organization and function in eukaryotic organisms.

PubMed: 28552577
DOI: 10.1016/j.str.2017.04.010

実験手法

X-RAY DIFFRACTION (2.3 Å)