5N5Z

Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation II)

5N5Z の概要

• エントリーDOI: 10.2210/pdb5n5z/pdb
• EMDBエントリー: 3591
• 分子名称: DNA-directed RNA polymerase I subunit RPA190, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, ... (19 entities in total)
• 機能のキーワード: transcription, rna polymerase i initiation
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 885192.75

構造登録者

Engel, C.,Gubbey, T.,Neyer, S.,Sainsbury, S.,Oberthuer, C.,Baejen, C.,Bernecky, C.,Cramer, P. (登録日: 2017-02-14, 公開日: 2017-04-05, 最終更新日: 2018-10-03)

主引用文献

Engel, C.,Gubbey, T.,Neyer, S.,Sainsbury, S.,Oberthuer, C.,Baejen, C.,Bernecky, C.,Cramer, P.
Structural Basis of RNA Polymerase I Transcription Initiation.
Cell, 169:120-131.e22, 2017

PubMed Abstract: Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct "bendability" and "meltability" of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase.

PubMed: 28340337
DOI: 10.1016/j.cell.2017.03.003

実験手法

ELECTRON MICROSCOPY (7.7 Å)