5N07

Structure of the [4Fe-4S] form of the NO response regulator NsrR

5N07 の概要

• エントリーDOI: 10.2210/pdb5n07/pdb
• 分子名称: HTH-type transcriptional repressor NsrR, IRON/SULFUR CLUSTER, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: no sensor, iron sulfur cluster, transcription
• 由来する生物種: Streptomyces coelicolor A3(2)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17986.26

構造登録者

Volbeda, A.,Fontecilla-Camps, J.C. (登録日: 2017-02-02, 公開日: 2017-04-26, 最終更新日: 2024-05-08)

主引用文献

Volbeda, A.,Dodd, E.L.,Darnault, C.,Crack, J.C.,Renoux, O.,Hutchings, M.I.,Le Brun, N.E.,Fontecilla-Camps, J.C.
Crystal structures of the NO sensor NsrR reveal how its iron-sulfur cluster modulates DNA binding.
Nat Commun, 8:15052-15052, 2017

PubMed Abstract: NsrR from Streptomyces coelicolor (Sc) regulates the expression of three genes through the progressive degradation of its [4Fe-4S] cluster on nitric oxide (NO) exposure. We report the 1.95 Å resolution crystal structure of dimeric holo-ScNsrR and show that the cluster is coordinated by the three invariant Cys residues from one monomer and, unexpectedly, Asp8 from the other. A cavity map suggests that NO displaces Asp8 as a cluster ligand and, while D8A and D8C variants remain NO sensitive, DNA binding is affected. A structural comparison of holo-ScNsrR with an apo-IscR-DNA complex shows that the [4Fe-4S] cluster stabilizes a turn between ScNsrR Cys93 and Cys99 properly oriented to interact with the DNA backbone. In addition, an apo ScNsrR structure suggests that Asn97 from this turn, along with Arg12, which forms a salt-bridge with Asp8, are instrumental in modulating the position of the DNA recognition helix region relative to its major groove.

PubMed: 28425466
DOI: 10.1038/ncomms15052

実験手法

X-RAY DIFFRACTION (1.95 Å)