5MYD

Convergent evolution involving dimeric and trimeric dUTPases in signalling.

5MYD の概要

• エントリーDOI: 10.2210/pdb5myd/pdb
• 分子名称: DUTPase, 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: staphylococcus aureus, pathogenicity island, sapi, dutpases, signalling, gene transfer, mobile genetic elements, hydrolase
• 由来する生物種: Staphylococcus sp. HMSC068C09
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 98687.49

構造登録者

Donderis, J.,Bowring, J.,Maiques, E.,Ciges-Tomas, J.R.,Alite, C.,Mehmedov, I.,Tormo-Mas, M.A.,Penades, J.R.,Marina, A. (登録日: 2017-01-26, 公開日: 2017-09-06, 最終更新日: 2024-05-08)

主引用文献

Donderis, J.,Bowring, J.,Maiques, E.,Ciges-Tomas, J.R.,Alite, C.,Mehmedov, I.,Tormo-Mas, M.A.,Penades, J.R.,Marina, A.
Convergent evolution involving dimeric and trimeric dUTPases in pathogenicity island mobilization.
PLoS Pathog., 13:e1006581-e1006581, 2017

PubMed Abstract: The dUTPase (Dut) enzymes, encoded by almost all free-living organisms and some viruses, prevent the misincorporation of uracil into DNA. We previously proposed that trimeric Duts are regulatory proteins involved in different cellular processes; including the phage-mediated transfer of the Staphylococcus aureus pathogenicity island SaPIbov1. Recently, it has been shown that the structurally unrelated dimeric Dut encoded by phage ϕNM1 is similarly able to mobilize SaPIbov1, suggesting dimeric Duts could also be regulatory proteins. How this is accomplished remains unsolved. Here, using in vivo, biochemical and structural approaches, we provide insights into the signaling mechanism used by the dimeric Duts to induce the SaPIbov1 cycle. As reported for the trimeric Duts, dimeric Duts contain an extremely variable region, here named domain VI, which is involved in the regulatory capacity of these enzymes. Remarkably, our results also show that the dimeric Dut signaling mechanism is modulated by dUTP, as with the trimeric Duts. Overall, our results demonstrate that although unrelated both in sequence and structure, dimeric and trimeric Duts control SaPI transfer by analogous mechanisms, representing a fascinating example of convergent evolution. This conserved mode of action highlights the biological significance of Duts as regulatory molecules.

PubMed: 28892519
DOI: 10.1371/journal.ppat.1006581

実験手法

X-RAY DIFFRACTION (2.3 Å)