5MY1

E. coli expressome

5MY1 の概要

• エントリーDOI: 10.2210/pdb5my1/pdb
• EMDBエントリー: 3580
• 分子名称: 16S ribosomal RNA, 30S ribosomal protein S11, 30S ribosomal protein S12, ... (26 entities in total)
• 機能のキーワード: expressome ribosome rna polymerase, ribosome
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 26
• 化学式量合計: 1177189.17

構造登録者

Kohler, R.,Mooney, R.A.,Mills, D.J.,Kostrewa, D.,Landick, R.,Cramer, P. (登録日: 2017-01-25, 公開日: 2017-04-26, 最終更新日: 2024-05-15)

主引用文献

Kohler, R.,Mooney, R.A.,Mills, D.J.,Landick, R.,Cramer, P.
Architecture of a transcribing-translating expressome.
Science, 356:194-197, 2017

PubMed Abstract: DNA transcription is functionally coupled to messenger RNA (mRNA) translation in bacteria, but how this is achieved remains unclear. Here we show that RNA polymerase (RNAP) and the ribosome of can form a defined transcribing and translating "expressome" complex. The cryo-electron microscopic structure of the expressome reveals continuous protection of ~30 nucleotides of mRNA extending from the RNAP active center to the ribosome decoding center. The RNAP-ribosome interface includes the RNAP subunit α carboxyl-terminal domain, which is required for RNAP-ribosome interaction in vitro and for pronounced cell growth defects upon translation inhibition in vivo, consistent with its function in transcription-translation coupling. The expressome structure can only form during transcription elongation and explains how translation can prevent transcriptional pausing, backtracking, and termination.

PubMed: 28408604
DOI: 10.1126/science.aal3059

実験手法

ELECTRON MICROSCOPY (7.6 Å)