5MX9

High resolution crystal structure of the MCR-2 catalytic domain

5MX9 の概要

• エントリーDOI: 10.2210/pdb5mx9/pdb
• 分子名称: Phosphatidylethanolamine transferase Mcr-2, ZINC ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: mcr-1, colistin, antibiotic resistance, transferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36488.33

構造登録者

Hinchliffe, P.,Coates, K.,Walsh, T.R.,Spencer, J. (登録日: 2017-01-22, 公開日: 2017-08-09, 最終更新日: 2024-10-23)

主引用文献

Coates, K.,Walsh, T.R.,Spencer, J.,Hinchliffe, P.
1.12 angstrom resolution crystal structure of the catalytic domain of the plasmid-mediated colistin resistance determinant MCR-2.
Acta Crystallogr F Struct Biol Commun, 73:443-449, 2017

PubMed Abstract: MCR-2 confers resistance to colistin, a `last-line' antibiotic against extensively resistant Gram-negative pathogens. It is a plasmid-encoded phosphoethanolamine transferase that is closely related to MCR-1. To understand the diversity in the MCR family, the 1.12 Å resolution crystal structure of the catalytic domain of MCR-2 was determined. Variable amino acids are located distant from both the di-zinc active site and the membrane-proximal face. The exceptionally high resolution will provide an accurate starting model for further mechanistic studies.

PubMed: 28777086
DOI: 10.1107/S2053230X17009669

実験手法

X-RAY DIFFRACTION (1.12 Å)