5MWI

Crystal structure of G(CUG)8G duplex

5MWI の概要

• エントリーDOI: 10.2210/pdb5mwi/pdb
• 分子名称: RNA G(CUG)8C duplex, SULFATE ION (3 entities in total)
• 機能のキーワード: cug repeats, rna, duplex
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8449.98

構造登録者

Kiliszek, A.,Blaszczyk, L.,Rypniewski, W. (登録日: 2017-01-18, 公開日: 2017-03-08, 最終更新日: 2024-01-17)

主引用文献

Kiliszek, A.,Blaszczyk, L.,Kierzek, R.,Rypniewski, W.
Stabilization of RNA hairpins using non-nucleotide linkers and circularization.
Nucleic Acids Res., 45:e92-e92, 2017

PubMed Abstract: An RNA hairpin is an essential structural element of RNA. Hairpins play crucial roles in gene expression and intermolecular recognition but are also involved in the pathogenesis of some congenital diseases. Structural studies of the hairpin motifs are impeded by their thermodynamic instability, as they tend to unfold to form duplexes, especially at high concentrations required for crystallography or nuclear magnetic resonance spectroscopy. We have elaborated techniques to stabilize the RNA hairpins by linking the free ends of the RNA strand at the base of the hairpin stem. One method involves stilbene diether or hexaethylene glycol linkers and circularization by T4 RNA ligase. Another method uses click chemistry to stitch the RNA ends with a triazole linker. Both techniques are efficient and easy to perform. They should be useful in making stable, biologically relevant RNA constructs for structural studies.

PubMed: 28334744
DOI: 10.1093/nar/gkx122

実験手法

X-RAY DIFFRACTION (1.51 Å)