5MW5

Human Jagged2 C2-EGF2

5MW5 の概要

• エントリーDOI: 10.2210/pdb5mw5/pdb
• 分子名称: Protein jagged-2, 2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: c2, egf, notch, signaling, signaling protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32894.84

構造登録者

Suckling, R.J.,Handford, P.A.,Lea, S.M. (登録日: 2017-01-18, 公開日: 2017-06-14, 最終更新日: 2024-01-17)

主引用文献

Suckling, R.J.,Korona, B.,Whiteman, P.,Chillakuri, C.,Holt, L.,Handford, P.A.,Lea, S.M.
Structural and functional dissection of the interplay between lipid and Notch binding by human Notch ligands.
EMBO J., 36:2204-2215, 2017

PubMed Abstract: Recent data have expanded our understanding of Notch signalling by identifying a C2 domain at the N-terminus of Notch ligands, which has both lipid- and receptor-binding properties. We present novel structures of human ligands Jagged2 and Delta-like4 and human Notch2, together with functional assays, which suggest that ligand-mediated coupling of membrane recognition and Notch binding is likely to be critical in establishing the optimal context for Notch signalling. Comparisons between the Jagged and Delta family show a huge diversity in the structures of the loops at the apex of the C2 domain implicated in membrane recognition and Jagged1 missense mutations, which affect these loops and are associated with extrahepatic biliary atresia, lead to a loss of membrane recognition, but do not alter Notch binding. Taken together, these data suggest that C2 domain binding to membranes is an important element in tuning ligand-dependent Notch signalling in different physiological contexts.

PubMed: 28572448
DOI: 10.15252/embj.201796632

実験手法

X-RAY DIFFRACTION (2.7 Å)