5MTA

G-quadruplex formed within promoters of Plasmodium falciparum B var genes

5MTA の概要

• エントリーDOI: 10.2210/pdb5mta/pdb
• NMR情報: BMRB: 34083
• 分子名称: UpsB-Q-1, DNA (34-MER) (1 entity in total)
• 機能のキーワード: g-quadruplex, nucleic acid, long loop, nmr spectroscopy, malaria, dna
• 由来する生物種: Plasmodium falciparum (malaria parasite P. falciparum)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10664.85

構造登録者

Juribasic Kulcsar, M.,Plavec, J. (登録日: 2017-01-09, 公開日: 2018-02-28, 最終更新日: 2025-07-30)

主引用文献

Juribasic Kulcsar, M.,Gabelica, V.,Plavec, J.
Solution-State Structure of a Long-Loop G-Quadruplex Formed Within Promoters of Plasmodium falciparum B var Genes.
Chemistry, 30:e202401190-e202401190, 2024

PubMed Abstract: We report the high-resolution NMR solution-state structure of an intramolecular G-quadruplex with a diagonal loop of ten nucleotides. The G-quadruplex is formed by a 34-nt DNA sequence, d[CAGTAGTATACTAGTAGT], named UpsB-Q-1. This sequence is found within promoters of the var genes of Plasmodium falciparum, which play a key role in malaria pathogenesis and evasion of the immune system. The [3+1]-hybrid G-quadruplex formed under physiologically relevant conditions exhibits a unique equilibrium between two structures, both stabilized by base stacking and non-canonical hydrogen bonding. Unique equilibrium of the two closely related 3D structures originates from a North-South repuckering of deoxyribose moiety of residue T27 in the lateral loop. Besides the 12 guanines involved in three G-quartets, most residues in loop regions are involved in interactions at both G-quartet-loop interfaces.

PubMed: 38647110
DOI: 10.1002/chem.202401190

実験手法

SOLUTION NMR