5MLH

Plantago Major multifunctional oxidoreductase in complex with 8-oxogeranial and NADP+

Summary for 5MLH

• Entry DOI: 10.2210/pdb5mlh/pdb
• Descriptor: Progesterone 5-beta-reductase, SODIUM ION, (2E,6E)-2,6-dimethylocta-2,6-dienedial, ... (7 entities in total)
• Functional Keywords: progesterone reductase, iridoid synthase, sdr, enzyme evolution, oxidoreductase
• Biological source: Plantago major (Common plantain)
• Total number of polymer chains: 1
• Total formula weight: 45178.71

Authors

Fellows, R.,Russo, C.M.,Lee, S.G.,Jez, J.M.,Chisholm, J.D.,Zubieta, C.,Nanao, M. (deposition date: 2016-12-06, release date: 2018-08-01, Last modification date: 2024-01-17)

Primary citation

Fellows, R.,Russo, C.M.,Silva, C.S.,Lee, S.G.,Jez, J.M.,Chisholm, J.D.,Zubieta, C.,Nanao, M.H.
A multisubstrate reductase from Plantago major: structure-function in the short chain reductase superfamily.
Sci Rep, 8:14796-14796, 2018

PubMed Abstract: The short chain dehydrogenase/reductase superfamily (SDR) is a large family of NAD(P)H-dependent enzymes found in all kingdoms of life. SDRs are particularly well-represented in plants, playing diverse roles in both primary and secondary metabolism. In addition, some plant SDRs are also able to catalyse a reductive cyclisation reaction critical for the biosynthesis of the iridoid backbone that contains a fused 5 and 6-membered ring scaffold. Mining the EST database of Plantago major, a medicinal plant that makes iridoids, we identified a putative 5β-progesterone reductase gene, PmMOR (P. major multisubstrate oxido-reductase), that is 60% identical to the iridoid synthase gene from Catharanthus roseus. The PmMOR protein was recombinantly expressed and its enzymatic activity assayed against three putative substrates, 8-oxogeranial, citral and progesterone. The enzyme demonstrated promiscuous enzymatic activity and was able to not only reduce progesterone and citral, but also to catalyse the reductive cyclisation of 8-oxogeranial. The crystal structures of PmMOR wild type and PmMOR mutants in complex with NADP or NAD and either 8-oxogeranial, citral or progesterone help to reveal the substrate specificity determinants and catalytic machinery of the protein. Site-directed mutagenesis studies were performed and provide a foundation for understanding the promiscuous activity of the enzyme.

PubMed: 30287897
DOI: 10.1038/s41598-018-32967-1

Experimental method

X-RAY DIFFRACTION (1.86 Å)