5MJW

Structure of Psb29 at 1.55A

5MJW の概要

• エントリーDOI: 10.2210/pdb5mjw/pdb
• 分子名称: Protein Thf1 (2 entities in total)
• 機能のキーワード: photosystem ii ftsh, photosynthesis
• 由来する生物種: Thermosynechococcus elongatus (strain BP-1)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27268.85

構造登録者

Murray, J.W.,Kozlo, A. (登録日: 2016-12-02, 公開日: 2017-08-23, 最終更新日: 2024-01-17)

主引用文献

Bec Kova, M.,Yu, J.,Krynicka, V.,Kozlo, A.,Shao, S.,Konik, P.,Komenda, J.,Murray, J.W.,Nixon, P.J.
Structure of Psb29/Thf1 and its association with the FtsH protease complex involved in photosystem II repair in cyanobacteria.
Philos. Trans. R. Soc. Lond., B, Biol. Sci., 372:-, 2017

PubMed Abstract: One strategy for enhancing photosynthesis in crop plants is to improve their ability to repair photosystem II (PSII) in response to irreversible damage by light. Despite the pivotal role of thylakoid-embedded FtsH protease complexes in the selective degradation of PSII subunits during repair, little is known about the factors involved in regulating FtsH expression. Here we show using the cyanobacterium sp. PCC 6803 that the Psb29 subunit, originally identified as a minor component of His-tagged PSII preparations, physically interacts with FtsH complexes and is required for normal accumulation of the FtsH2/FtsH3 hetero-oligomeric complex involved in PSII repair. We show using X-ray crystallography that Psb29 from has a unique fold consisting of a helical bundle and an extended C-terminal helix and contains a highly conserved region that might be involved in binding to FtsH. A similar interaction is likely to occur in Arabidopsis chloroplasts between the Psb29 homologue, termed THF1, and the FTSH2/FTSH5 complex. The direct involvement of Psb29/THF1 in FtsH accumulation helps explain why THF1 is a target during the hypersensitive response in plants induced by pathogen infection. Downregulating FtsH function and the PSII repair cycle via THF1 would contribute to the production of reactive oxygen species, the loss of chloroplast function and cell death.This article is part of the themed issue 'Enhancing photosynthesis in crop plants: targets for improvement'.

PubMed: 28808107
DOI: 10.1098/rstb.2016.0394

実験手法

X-RAY DIFFRACTION (2.47 Å)