5MIK

X-ray structure of carboplatin-encapsulated horse spleen apoferritin (rotating anode data)

5MIK の概要

• エントリーDOI: 10.2210/pdb5mik/pdb
• 分子名称: Ferritin light chain, CADMIUM ION, CHLORIDE ION, ... (7 entities in total)
• 機能のキーワード: metal transport
• 由来する生物種: Equus caballus (Horse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21885.71

構造登録者

Pontillo, N.,Ferraro, G.,Helliwell, J.R.,Merlino, A. (登録日: 2016-11-28, 公開日: 2017-03-15, 最終更新日: 2024-01-17)

主引用文献

Pontillo, N.,Ferraro, G.,Helliwell, J.R.,Amoresano, A.,Merlino, A.
X-ray Structure of the Carboplatin-Loaded Apo-Ferritin Nanocage.
ACS Med Chem Lett, 8:433-437, 2017

PubMed Abstract: The second-generation Pt anticancer agent carboplatin (CBDCA) was encapsulated within the apo horse spleen ferritin (AFt) nanocage, and the X-ray structure of the drug-loaded protein was refined at 1.49 Å resolution. Two Pt binding sites, different from the one observed in the cisplatin-encapsulated AFt, were identified in Ft subunits by inspection of anomalous electron density maps at two wavelengths and difference Fourier electron density maps, which provide the necessary sensitivity to discriminate between Pt from CBDCA and Cd ions that are present in the crystallization conditions. Pt centers coordinate to the NE2 atom of His49 and to the NE2 atom of His132, both on the inner surface of the Ft nanocage.

PubMed: 28435532
DOI: 10.1021/acsmedchemlett.7b00025

実験手法

X-RAY DIFFRACTION (1.96 Å)