5MIH

Crystal structure of the lectin LecA from Pseudomonas aeruginosa in complex with a phenyl-epoxy-galactopyranoside

5MIH の概要

• エントリーDOI: 10.2210/pdb5mih/pdb
• 分子名称: PA-I galactophilic lectin, CALCIUM ION, phenyl 6,7-anhydro-D-glycero-beta-D-galacto-heptopyranoside, ... (5 entities in total)
• 機能のキーワード: lectin, galactose binding protein, covalent lectin inhibition, sugar binding protein
• 由来する生物種: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
• 細胞内の位置: Cytoplasm: Q05097
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 52522.16

構造登録者

Wagner, S.,Hauk, D.,Hofmann, M.,Joachim, I.,Sommer, R.,Muller, R.,Imberty, A.,Varrot, A.,Titz, A. (登録日: 2016-11-28, 公開日: 2017-10-11, 最終更新日: 2024-01-17)

主引用文献

Wagner, S.,Hauck, D.,Hoffmann, M.,Sommer, R.,Joachim, I.,Muller, R.,Imberty, A.,Varrot, A.,Titz, A.
Covalent Lectin Inhibition and Application in Bacterial Biofilm Imaging.
Angew. Chem. Int. Ed. Engl., 56:16559-16564, 2017

PubMed Abstract: Biofilm formation by pathogenic bacteria is a hallmark of chronic infections. In many cases, lectins play key roles in establishing biofilms. The pathogen Pseudomonas aeruginosa often exhibiting various drug resistances employs its lectins LecA and LecB as virulence factors and biofilm building blocks. Therefore, inhibition of the function of these proteins is thought to have potential in developing "pathoblockers" preventing biofilm formation and virulence. A covalent lectin inhibitor specific to a carbohydrate binding site is described for the first time. Its application in the LecA-specific in vitro imaging of biofilms formed by P. aeruginosa is also reported.

PubMed: 28960731
DOI: 10.1002/anie.201709368

実験手法

X-RAY DIFFRACTION (1.8 Å)