5MHD

Biosynthetic engineered A22S-B3K-B31R human insulin monomer structure in water/acetonitrile solutions.

5MHD の概要

• エントリーDOI: 10.2210/pdb5mhd/pdb
• NMR情報: BMRB: 34070
• 分子名称: Insulin (2 entities in total)
• 機能のキーワード: human insulin, water/acetonitrile solution, mutant, hormone
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6077.00

構造登録者

Bocian, W.,Kozerski, L.,Bednarek, E.,Sitkowski, J. (登録日: 2016-11-24, 公開日: 2017-08-09, 最終更新日: 2024-11-13)

主引用文献

Bednarek, E.,Sitkowski, J.,Bocian, W.,Borowicz, P.,Pucienniczak, G.,Stadnik, D.,Surmacz-Chwedoruk, W.,Jaworska, B.,Kozerski, L.
Structure and pharmaceutical formulation development of a new long-acting recombinant human insulin analog studied by NMR and MS.
J Pharm Biomed Anal, 135:126-132, 2017

PubMed Abstract: A monomer structure of a novel human insulin analog A22-B3-B31 (SK3R) has been characterized by NMR in water/acetonitrile solution and compared with the structure of human insulin (HIS) established in the same medium. The composition of the oligomer ensemble for neat insulins in water was qualitatively assessed by monitoring, derived from NMR experiment, translational diffusion coefficient Dix10ms, whose value is a population averaged of individual coefficients for species in oligomeric ensemble. Nanospray ESI/MS experiment was used to establish the masses of oligomers in pharmaceutical formulation of the SK3R insulin. The pharmacodynamic data were established and compared to insulin glargine characterized by the same profile of action in diabetics. The oligomerization process of insulin during development of pharmaceutical formulation with routinely used excipients has been studied using translation diffusion coefficient Dix10ms established in water solution. These properties were compared with those of human insulin (HIS) which is a standard reference for novel recombinant insulins.

PubMed: 28024260
DOI: 10.1016/j.jpba.2016.12.005

実験手法

SOLUTION NMR