5MG0

Structure of PAS-GAF fragment of Deinococcus phytochrome by serial femtosecond crystallography

5MG0 の概要

• エントリーDOI: 10.2210/pdb5mg0/pdb
• 関連するPDBエントリー: 2O9C 4Q0H 4Y3I 4Y5F
• 分子名称: Bacteriophytochrome, 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid, NICKEL (II) ION, ... (6 entities in total)
• 機能のキーワード: phytochrome, photoreceptor, bilin, sfx, transferase
• 由来する生物種: Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37801.59

構造登録者

Burgie, E.S.,Fuller, F.D.,Gul, S.,Miller, M.D.,Young, I.D.,Brewster, A.S.,Clinger, J.,Aller, P.,Braeuer, P.,Hutchison, C.,Alonso-Mori, R.,Kern, J.,Yachandra, V.K.,Yano, J.,Sauter, N.K.,Phillips Jr., G.N.,Vierstra, R.D.,Orville, A.M. (登録日: 2016-11-20, 公開日: 2017-02-22, 最終更新日: 2024-11-20)

主引用文献

Fuller, F.D.,Gul, S.,Chatterjee, R.,Burgie, E.S.,Young, I.D.,Lebrette, H.,Srinivas, V.,Brewster, A.S.,Michels-Clark, T.,Clinger, J.A.,Andi, B.,Ibrahim, M.,Pastor, E.,de Lichtenberg, C.,Hussein, R.,Pollock, C.J.,Zhang, M.,Stan, C.A.,Kroll, T.,Fransson, T.,Weninger, C.,Kubin, M.,Aller, P.,Lassalle, L.,Brauer, P.,Miller, M.D.,Amin, M.,Koroidov, S.,Roessler, C.G.,Allaire, M.,Sierra, R.G.,Docker, P.T.,Glownia, J.M.,Nelson, S.,Koglin, J.E.,Zhu, D.,Chollet, M.,Song, S.,Lemke, H.,Liang, M.,Sokaras, D.,Alonso-Mori, R.,Zouni, A.,Messinger, J.,Bergmann, U.,Boal, A.K.,Bollinger, J.M.,Krebs, C.,Hogbom, M.,Phillips, G.N.,Vierstra, R.D.,Sauter, N.K.,Orville, A.M.,Kern, J.,Yachandra, V.K.,Yano, J.
Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers.
Nat. Methods, 14:443-449, 2017

PubMed Abstract: X-ray crystallography at X-ray free-electron laser sources is a powerful method for studying macromolecules at biologically relevant temperatures. Moreover, when combined with complementary techniques like X-ray emission spectroscopy, both global structures and chemical properties of metalloenzymes can be obtained concurrently, providing insights into the interplay between the protein structure and dynamics and the chemistry at an active site. The implementation of such a multimodal approach can be compromised by conflicting requirements to optimize each individual method. In particular, the method used for sample delivery greatly affects the data quality. We present here a robust way of delivering controlled sample amounts on demand using acoustic droplet ejection coupled with a conveyor belt drive that is optimized for crystallography and spectroscopy measurements of photochemical and chemical reactions over a wide range of time scales. Studies with photosystem II, the phytochrome photoreceptor, and ribonucleotide reductase R2 illustrate the power and versatility of this method.

PubMed: 28250468
DOI: 10.1038/nmeth.4195

実験手法

X-RAY DIFFRACTION (1.65 Å)