5MDW

Structure of ArfA(A18T) and RF2 bound to the 70S ribosome (pre-accommodated state)

これはPDB形式変換不可エントリーです。

5MDW の概要

• エントリーDOI: 10.2210/pdb5mdw/pdb
• EMDBエントリー: 3490
• 分子名称: 23S ribosomal RNA, 50S ribosomal protein L4, 50S ribosomal protein L5, ... (62 entities in total)
• 機能のキーワード: ribosome, arfa rf2 trans-translation, 70s, 50s, 30s, rescue termination, cryo-em
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 58
• 化学式量合計: 2260975.29

構造登録者

James, N.R.,Brown, A.,Gordiyenko, Y.,Ramakrishnan, V. (登録日: 2016-11-13, 公開日: 2016-12-14, 最終更新日: 2024-04-24)

主引用文献

James, N.R.,Brown, A.,Gordiyenko, Y.,Ramakrishnan, V.
Translational termination without a stop codon.
Science, 354:1437-1440, 2016

PubMed Abstract: Ribosomes stall when they encounter the end of messenger RNA (mRNA) without an in-frame stop codon. In bacteria, these "nonstop" complexes can be rescued by alternative ribosome-rescue factor A (ArfA). We used electron cryomicroscopy to determine structures of ArfA bound to the ribosome with 3'-truncated mRNA, at resolutions ranging from 3.0 to 3.4 angstroms. ArfA binds within the ribosomal mRNA channel and substitutes for the absent stop codon in the A site by specifically recruiting release factor 2 (RF2), initially in a compact preaccommodated state. A similar conformation of RF2 may occur on stop codons, suggesting a general mechanism for release-factor-mediated translational termination in which a conformational switch leads to peptide release only when the appropriate signal is present in the A site.

PubMed: 27934701
DOI: 10.1126/science.aai9127

実験手法

ELECTRON MICROSCOPY (3.06 Å)