5MDJ

Crystal structure of an O2-tolerant [NiFe]-hydrogenase from Ralstonia eutropha in a its as-isolated high-pressurized form

Summary for 5MDJ

• Entry DOI: 10.2210/pdb5mdj/pdb
• Descriptor: Uptake hydrogenase large subunit; HOXG, Uptake hydrogenase small subunit; HOXK, NI-FE OXIDIZED ACTIVE CENTER, ... (9 entities in total)
• Functional Keywords: [nife] hydrogenase, knallgasbacteria, proteobacteria, aerobic hydrogen bacteria, high pressure pumping, dehydrogenase, oxidoreductase, hydrogen catalysis, metalloenzyme, dihydrogen, bimetallic, metalloprotein catalytic center, ni-fe active site, t-cluster, fe-s cluster, [4fe-3s] cluster, [3fe-4s] cluster, [4fe-4s] cluster, as-isolated state, oxidized state, oxygen tolerant hydrogenase, membrane bound, membrane, hydrophobic tunnel, high pressure cryo cooling, gas transport
• Biological source: Ralstonia eutropha H16; More
• Total number of polymer chains: 2
• Total formula weight: 104511.57

Authors

Schmidt, A.,Kalms, J.,Scheerer, P. (deposition date: 2016-11-11, release date: 2018-02-21, Last modification date: 2024-01-17)

• Primary citation: Kalms, J.,Schmidt, A.,Frielingsdorf, S.,Utesch, T.,Gotthard, G.,von Stetten, D.,van der Linden, P.,Royant, A.,Mroginski, M.A.,Carpentier, P.,Lenz, O.,Scheerer, P.; Tracking the route of molecular oxygen in O2-tolerant membrane-bound [NiFe] hydrogenase.; Proc. Natl. Acad. Sci. U.S.A., 115:E2229-E2237, 2018; PubMed: 29463722; DOI: 10.1073/pnas.1712267115

Experimental method

X-RAY DIFFRACTION (1.48 Å)