5M5T

Clathrin heavy chain N-terminal domain bound to a non-natural clathrin-box motif peptide (Amph4T1)

5M5T の概要

• エントリーDOI: 10.2210/pdb5m5t/pdb
• 分子名称: Clathrin heavy chain 1, Amphiphysin, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: endocytosis
• 由来する生物種: Bos taurus (Bovine); 詳細
• 細胞内の位置: Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side: P49951; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side : O08838
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 88417.00

構造登録者

Muenzner, J.,Graham, S.C. (登録日: 2016-10-22, 公開日: 2016-11-16, 最終更新日: 2024-01-17)

主引用文献

Muenzner, J.,Traub, L.M.,Kelly, B.T.,Graham, S.C.
Cellular and viral peptides bind multiple sites on the N-terminal domain of clathrin.
Traffic, 18:44-57, 2017

PubMed Abstract: Short peptide motifs in unstructured regions of clathrin-adaptor proteins recruit clathrin to membranes to facilitate post-Golgi membrane transport. Three consensus clathrin-binding peptide sequences have been identified and structural studies show that each binds distinct sites on the clathrin heavy chain N-terminal domain (NTD). A fourth binding site for adaptors on NTD has been functionally identified but not structurally characterised. We have solved high resolution structures of NTD bound to peptide motifs from the cellular clathrin adaptors β2 adaptin and amphiphysin plus a putative viral clathrin adaptor, hepatitis D virus large antigen (HDAg-L). Surprisingly, with each peptide we observe simultaneous peptide binding at multiple sites on NTD and viral peptides binding to the same sites as cellular peptides. Peptides containing clathrin-box motifs (CBMs) with the consensus sequence LΦxΦ[DE] bind at the 'arrestin box' on NTD, between β-propeller blades 4 and 5, which had previously been thought to bind a distinct consensus sequence. Further, we structurally define the fourth peptide binding site on NTD, which we term the Royle box. In vitro binding assays show that clathrin is more readily captured by cellular CBMs than by HDAg-L, and site-directed mutagenesis confirms that multiple binding sites on NTD contribute to efficient capture by CBM peptides.

PubMed: 27813245
DOI: 10.1111/tra.12457

実験手法

X-RAY DIFFRACTION (1.7 Å)