5M3S

Low-dose fixed target serial synchrotron crystallography structure of Metmyoglobin

Summary for 5M3S

• Entry DOI: 10.2210/pdb5m3s/pdb
• Descriptor: Myoglobin, PROTOPORPHYRIN IX CONTAINING FE, SULFATE ION, ... (4 entities in total)
• Functional Keywords: oxygen storage, oxygen binding
• Biological source: Physeter catodon (Sperm whale); More
• Total number of polymer chains: 1
• Total formula weight: 18365.90

Authors

Axford, D.,Owen, R.L.,Sherrell, D.,Muller-Werkmeister, H. (deposition date: 2016-10-17, release date: 2016-12-14, Last modification date: 2024-01-17)

Primary citation

Owen, R.L.,Axford, D.,Sherrell, D.A.,Kuo, A.,Ernst, O.P.,Schulz, E.C.,Miller, R.J.,Mueller-Werkmeister, H.M.
Low-dose fixed-target serial synchrotron crystallography.
Acta Crystallogr D Struct Biol, 73:373-378, 2017

PubMed Abstract: The development of serial crystallography has been driven by the sample requirements imposed by X-ray free-electron lasers. Serial techniques are now being exploited at synchrotrons. Using a fixed-target approach to high-throughput serial sampling, it is demonstrated that high-quality data can be collected from myoglobin crystals, allowing room-temperature, low-dose structure determination. The combination of fixed-target arrays and a fast, accurate translation system allows high-throughput serial data collection at high hit rates and with low sample consumption.

PubMed: 28375148
DOI: 10.1107/S2059798317002996

Experimental method

X-RAY DIFFRACTION (1.8 Å)