5M2B

Yeast 20S proteasome with human beta5i (1-138) and human beta6 (97-111; 118-133) in complex with thiazole based inhibitor Ro19

5M2B の概要

• エントリーDOI: 10.2210/pdb5m2b/pdb
• 関連するPDBエントリー: 5L5B
• 分子名称: Proteasome subunit alpha type-2, Proteasome subunit beta type-4, Proteasome subunit beta type-8,Proteasome subunit beta type-5, ... (18 entities in total)
• 機能のキーワード: hydrolase-hydrolase inhibitor complex, proteasome, mutant, inhibitor, binding analysis, hydrolase/hydrolase inhibitor
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 732598.31

構造登録者

Groll, M. (登録日: 2016-10-12, 公開日: 2017-01-25, 最終更新日: 2024-01-17)

主引用文献

Cui, H.,Baur, R.,Le Chapelain, C.,Dubiella, C.,Heinemeyer, W.,Huber, E.M.,Groll, M.
Structural Elucidation of a Nonpeptidic Inhibitor Specific for the Human Immunoproteasome.
Chembiochem, 18:523-526, 2017

PubMed Abstract: Selective inhibition of the immunoproteasome is a promising approach towards the development of immunomodulatory drugs. Recently, a class of substituted thiazole compounds that combine a nonpeptidic scaffold with the absence of an electrophile was reported in a patent. Here, we investigated the mode of action of the lead compound by using a sophisticated chimeric yeast model of the human immunoproteasome for structural studies. The inhibitor adopts a unique orientation perpendicular to the β5i substrate-binding channel. Distinct interactions between the inhibitor and the subpockets of the human immunoproteasome account for its isotype selectivity.

PubMed: 28098422
DOI: 10.1002/cbic.201700021

実験手法

X-RAY DIFFRACTION (2.7 Å)