5M0T

Alpha-ketoglutarate-dependent non-heme iron oxygenase EasH

Summary for 5M0T

• Entry DOI: 10.2210/pdb5m0t/pdb
• Descriptor: Alpha-ketoglutarate-dependent non-heme iron oxygenase EasH, FE (II) ION, 2-OXOGLUTARIC ACID, ... (5 entities in total)
• Functional Keywords: cycloclavine biosynthesis, eash, alpha-ketoglutarate-dependent non-heme iron oxygenase, ergot alkaloids, oxidoreductase
• Biological source: Aspergillus japonicus
• Total number of polymer chains: 2
• Total formula weight: 65987.53

Authors

Jakubczyk, D.,Caputi, L.,Stevenson, C.E.M.,Lawson, D.M.,O'Connor, S.E. (deposition date: 2016-10-05, release date: 2016-12-07, Last modification date: 2024-01-17)

Primary citation

Jakubczyk, D.,Caputi, L.,Stevenson, C.E.,Lawson, D.M.,O'Connor, S.E.
Structural characterization of EasH (Aspergillus japonicus) - an oxidase involved in cycloclavine biosynthesis.
Chem. Commun. (Camb.), 52:14306-14309, 2016

PubMed Abstract: Aj_EasH is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. The three dimensional structure of Aj_EasH (2.2 Å resolution) reported here provides insight into the mechanism of this unusual and complex reaction.

PubMed: 27885368
DOI: 10.1039/c6cc08438a

Experimental method

X-RAY DIFFRACTION (2.2 Å)