5LZ3

Crystal structure of human ACBD3 GOLD domain in complex with 3A protein of Aichivirus A

5LZ3 の概要

• エントリーDOI: 10.2210/pdb5lz3/pdb
• 分子名称: Golgi resident protein GCP60, 3A (2 entities in total)
• 機能のキーワード: acbd3, gold, 3a, aichivirus, antiviral protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Golgi apparatus membrane ; Peripheral membrane protein ; Cytoplasmic side : Q9H3P7; Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Virion : O91464
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 25873.68

構造登録者

Klima, M.,Boura, E. (登録日: 2016-09-29, 公開日: 2016-12-14, 最終更新日: 2024-01-17)

主引用文献

Klima, M.,Chalupska, D.,Rozycki, B.,Humpolickova, J.,Rezabkova, L.,Silhan, J.,Baumlova, A.,Dubankova, A.,Boura, E.
Kobuviral Non-structural 3A Proteins Act as Molecular Harnesses to Hijack the Host ACBD3 Protein.
Structure, 25:219-230, 2017

PubMed Abstract: Picornaviruses are small positive-sense single-stranded RNA viruses that include many important human pathogens. Within the host cell, they replicate at specific replication sites called replication organelles. To create this membrane platform, they hijack several host factors including the acyl-CoA-binding domain-containing protein-3 (ACBD3). Here, we present a structural characterization of the molecular complexes formed by the non-structural 3A proteins from two species of the Kobuvirus genus of the Picornaviridae family and the 3A-binding domain of the host ACBD3 protein. Specifically, we present a series of crystal structures as well as a molecular dynamics simulation of the 3A:ACBD3 complex at the membrane, which reveals that the viral 3A proteins act as molecular harnesses to enslave the ACBD3 protein leading to its stabilization at target membranes. Our data provide a structural rationale for understanding how these viral-host protein complexes assemble at the atomic level and identify new potential targets for antiviral therapies.

PubMed: 28065508
DOI: 10.1016/j.str.2016.11.021

実験手法

X-RAY DIFFRACTION (3 Å)