5LZ0

Llama nanobody PorM_01

5LZ0 の概要

• エントリーDOI: 10.2210/pdb5lz0/pdb
• 分子名称: PorM nanobody (2 entities in total)
• 機能のキーワード: nanobody, porphyromonas gingivalis, type-9 secretion system (t9ss), immune system
• 由来する生物種: Lama glama (Llama)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29991.05

構造登録者

Duhoo, Y.,Leone, P.,Roussel, A. (登録日: 2016-09-29, 公開日: 2017-05-03, 最終更新日: 2024-01-17)

主引用文献

Duhoo, Y.,Roche, J.,Trinh, T.T.N.,Desmyter, A.,Gaubert, A.,Kellenberger, C.,Cambillau, C.,Roussel, A.,Leone, P.
Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis.
Acta Crystallogr F Struct Biol Commun, 73:286-293, 2017

PubMed Abstract: PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. In the context of structural studies of PorM to better understand T9SS assembly, four camelid nanobodies were selected, produced and purified, and their specific interaction with the N-terminal or C-terminal part of the periplasmic domain of PorM was investigated. Diffracting crystals were also obtained, and the structures of the four nanobodies were solved by molecular replacement. Furthermore, two nanobodies were used as crystallization chaperones and turned out to be valuable tools in the structure-determination process of the periplasmic domain of PorM.

PubMed: 28471361
DOI: 10.1107/S2053230X17005969

実験手法

X-RAY DIFFRACTION (1.6 Å)