5LXR

Structure of the minimal RBM7 - ZCCHC8 Complex

5LXR の概要

• エントリーDOI: 10.2210/pdb5lxr/pdb
• 分子名称: RNA-binding protein 7, Zinc finger CCHC domain-containing protein 8, SAMARIUM (III) ION, ... (6 entities in total)
• 機能のキーワード: next complex rrm rbm7 zcchc8, rna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus, nucleoplasm : Q6NZY4
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15936.72

構造登録者

Falk, S.,Finogenova, K.,Benda, C.,Conti, E. (登録日: 2016-09-22, 公開日: 2016-12-14, 最終更新日: 2024-05-08)

主引用文献

Falk, S.,Finogenova, K.,Melko, M.,Benda, C.,Lykke-Andersen, S.,Jensen, T.H.,Conti, E.
Structure of the RBM7-ZCCHC8 core of the NEXT complex reveals connections to splicing factors.
Nat Commun, 7:13573-13573, 2016

PubMed Abstract: The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) complex, which directs a subset of non-coding RNAs for exosomal degradation. Here we elucidate how RBM7 is incorporated in the NEXT complex. We identify a proline-rich segment of ZCCHC8 as the interaction site for the RNA-recognition motif (RRM) of RBM7 and present the crystal structure of the corresponding complex at 2.0 Å resolution. On the basis of the structure, we identify a proline-rich segment within the splicing factor SAP145 with strong similarity to ZCCHC8. We show that this segment of SAP145 not only binds the RRM region of another splicing factor SAP49 but also the RRM of RBM7. These dual interactions of RBM7 with the exosome and the spliceosome suggest a model whereby NEXT might recruit the exosome to degrade intronic RNAs.

PubMed: 27905398
DOI: 10.1038/ncomms13573

実験手法

X-RAY DIFFRACTION (2 Å)