5LWT

Endothiapepsin in complex with a methoxylated derivative of fragment 177

5LWT の概要

• エントリーDOI: 10.2210/pdb5lwt/pdb
• 分子名称: Endothiapepsin, ACETATE ION, GLYCEROL, ... (7 entities in total)
• 機能のキーワード: fragment screening, aspartic protease, inhibition, reactivity, hydrolase
• 由来する生物種: Cryphonectria parasitica (Chestnut blight fungus)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34925.15

構造登録者

Schiebel, J.,Heine, A.,Klebe, G. (登録日: 2016-09-19, 公開日: 2017-08-09, 最終更新日: 2024-11-20)

主引用文献

Cramer, J.,Schiebel, J.,Wulsdorf, T.,Grohe, K.,Najbauer, E.E.,Ehrmann, F.R.,Radeva, N.,Zitzer, N.,Linne, U.,Linser, R.,Heine, A.,Klebe, G.
A False-Positive Screening Hit in Fragment-Based Lead Discovery: Watch out for the Red Herring.
Angew. Chem. Int. Ed. Engl., 56:1908-1913, 2017

PubMed Abstract: With the rising popularity of fragment-based approaches in drug development, more and more attention has to be devoted to the detection of false-positive screening results. In particular, the small size and low affinity of fragments drives screening techniques to their limit. The pursuit of a false-positive hit can cause significant loss of time and resources. Here, we present an instructive and intriguing investigation into the origin of misleading assay results for a fragment that emerged as the most potent binder for the aspartic protease endothiapepsin (EP) across multiple screening assays. This molecule shows its biological effect mainly after conversion into another entity through a reaction cascade that involves major rearrangements of its heterocyclic scaffold. The formed ligand binds EP through an induced-fit mechanism involving remarkable electrostatic interactions. Structural information in the initial screening proved to be crucial for the identification of this false-positive hit.

PubMed: 28097765
DOI: 10.1002/anie.201609824

実験手法

X-RAY DIFFRACTION (1.069 Å)