5LW7

S. solfataricus ABCE1 post-splitting state

5LW7 の概要

• エントリーDOI: 10.2210/pdb5lw7/pdb
• 関連するPDBエントリー: 4V6U
• EMDBエントリー: 4113
• 分子名称: ABC transporter ATP-binding protein, IRON/SULFUR CLUSTER (2 entities in total)
• 機能のキーワード: abce1, recycling, 30s, ribosome
• 由来する生物種: Pyrococcus abyssi (strain GE5 / Orsay)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 67989.55

構造登録者

Heuer, A.,Gerovac, M.,Beckmann, R.,Tampe, R. (登録日: 2016-09-15, 公開日: 2016-11-16, 最終更新日: 2024-11-13)

主引用文献

Kiosze-Becker, K.,Ori, A.,Gerovac, M.,Heuer, A.,Nurenberg-Goloub, E.,Rashid, U.J.,Becker, T.,Beckmann, R.,Beck, M.,Tampe, R.
Structure of the ribosome post-recycling complex probed by chemical cross-linking and mass spectrometry.
Nat Commun, 7:13248-13248, 2016

PubMed Abstract: Ribosome recycling orchestrated by the ATP binding cassette (ABC) protein ABCE1 can be considered as the final-or the first-step within the cyclic process of protein synthesis, connecting translation termination and mRNA surveillance with re-initiation. An ATP-dependent tweezer-like motion of the nucleotide-binding domains in ABCE1 transfers mechanical energy to the ribosome and tears the ribosome subunits apart. The post-recycling complex (PRC) then re-initiates mRNA translation. Here, we probed the so far unknown architecture of the 1-MDa PRC (40S/30S·ABCE1) by chemical cross-linking and mass spectrometry (XL-MS). Our study reveals ABCE1 bound to the translational factor-binding (GTPase) site with multiple cross-link contacts of the helix-loop-helix motif to the S24e ribosomal protein. Cross-linking of the FeS cluster domain to the ribosomal protein S12 substantiates an extreme lever-arm movement of the FeS cluster domain during ribosome recycling. We were thus able to reconstitute and structurally analyse a key complex in the translational cycle, resembling the link between translation initiation and ribosome recycling.

PubMed: 27824037
DOI: 10.1038/ncomms13248

実験手法

ELECTRON MICROSCOPY (17 Å)