5LUF

Cryo-EM of bovine respirasome

5LUF の概要

• エントリーDOI: 10.2210/pdb5luf/pdb
• EMDBエントリー: 4107
• 分子名称: Cytochrome b-c1 complex subunit 1, mitochondrial, Cytochrome b-c1 complex subunit 9, Cytochrome b-c1 complex subunit 10, ... (59 entities in total)
• 機能のキーワード: mitochondria, supercomplex, respiratory chain, oxidoreductase
• 由来する生物種: Bos taurus (Bovine); 詳細
• タンパク質・核酸の鎖数: 62
• 化学式量合計: 1293355.75

構造登録者

Sousa, J.S.,Mills, D.J.,Vonck, J.,Kuehlbrandt, W. (登録日: 2016-09-08, 公開日: 2016-11-30, 最終更新日: 2024-11-13)

主引用文献

Sousa, J.S.,Mills, D.J.,Vonck, J.,Kuhlbrandt, W.
Functional asymmetry and electron flow in the bovine respirasome.
Elife, 5:-, 2016

PubMed Abstract: Respirasomes are macromolecular assemblies of the respiratory chain complexes I, III and IV in the inner mitochondrial membrane. We determined the structure of supercomplex IIIIIV from bovine heart mitochondria by cryo-EM at 9 Å resolution. Most protein-protein contacts between complex I, III and IV in the membrane are mediated by supernumerary subunits. Of the two Rieske iron-sulfur cluster domains in the complex III dimer, one is resolved, indicating that this domain is immobile and unable to transfer electrons. The central position of the active complex III monomer between complex I and IV in the respirasome is optimal for accepting reduced quinone from complex I over a short diffusion distance of 11 nm, and delivering reduced cytochrome to complex IV. The functional asymmetry of complex III provides strong evidence for directed electron flow from complex I to complex IV through the active complex III monomer in the mammalian supercomplex.

PubMed: 27830641
DOI: 10.7554/eLife.21290

実験手法

ELECTRON MICROSCOPY (9.1 Å)