5LS8

Light-activated ruthenium complex bound to a DNA quadruplex

5LS8 の概要

• エントリーDOI: 10.2210/pdb5ls8/pdb
• 分子名称: DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*A)-3'), lambda-[Ru(tap2-dppz-CN)]2+, delta-[Ru(tap2-dppz-CN)]2+, ... (5 entities in total)
• 機能のキーワード: dna, quadruplex, chiral recognition, ruthenium
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 14641.25

構造登録者

McQuaid, K.T.,Abell, H.,Hall, J.P.,Cardin, C.J. (登録日: 2016-08-22, 公開日: 2018-01-17, 最終更新日: 2024-01-10)

主引用文献

McQuaid, K.,Abell, H.,Gurung, S.P.,Allan, D.R.,Winter, G.,Sorensen, T.,Cardin, D.J.,Brazier, J.A.,Cardin, C.J.,Hall, J.P.
Structural Studies Reveal Enantiospecific Recognition of a DNA G-Quadruplex by a Ruthenium Polypyridyl Complex.
Angew.Chem.Int.Ed.Engl., 58:9881-9885, 2019

PubMed Abstract: By using X-ray crystallography, we show that the complexes Λ/Δ-[Ru(TAP) (11-CN-dppz)] (TAP=1,4,5,8-tetraazaphenanthrene, dppz=dipyridophenazine) bind DNA G-quadruplex in an enantiospecific manner that parallels the specificity of these complexes with duplex DNA. The Λ complex crystallises with the normally parallel stranded d(TAGGGTTA) tetraplex to give the first such antiparallel strand assembly in which syn-guanosine is adjacent to the complex at the 5' end of the quadruplex core. SRCD measurements confirm that the same conformational switch occurs in solution. The Δ enantiomer, by contrast, is present in the structure but stacked at the ends of the assembly. In addition, we report the structure of Λ-[Ru(phen) (11-CN-dppz)] bound to d(TCGGCGCCGA), a duplex-forming sequence, and use both structural models to provide insight into the motif-specific luminescence response of the isostructural phen analogue enantiomers.

PubMed: 30958918
DOI: 10.1002/anie.201814502

実験手法

X-RAY DIFFRACTION (1.78 Å)