5LMV

Structure of bacterial 30S-IF1-IF2-IF3-mRNA-tRNA translation pre-initiation complex(state-III)

Summary for 5LMV

• Entry DOI: 10.2210/pdb5lmv/pdb
• EMDB information: 4083
• Descriptor: 16S ribosomal RNA, 30S ribosomal protein S10, 30S ribosomal protein S11, ... (29 entities in total)
• Functional Keywords: ribosome, translation, initiation factors, 30s, if1, if2, if3, trnai, pic, thermus thermophilus
• Biological source: Thermus thermophilus HB8; More
• Total number of polymer chains: 26
• Total formula weight: 913496.63

Authors

Hussain, T.,Llacer, J.L.,Wimberly, B.T.,Ramakrishnan, V. (deposition date: 2016-08-01, release date: 2016-10-05, Last modification date: 2024-10-16)

Primary citation

Hussain, T.,Llacer, J.L.,Wimberly, B.T.,Kieft, J.S.,Ramakrishnan, V.
Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation.
Cell, 167:133-144.e13, 2016

PubMed Abstract: In bacterial translational initiation, three initiation factors (IFs 1-3) enable the selection of initiator tRNA and the start codon in the P site of the 30S ribosomal subunit. Here, we report 11 single-particle cryo-electron microscopy (cryoEM) reconstructions of the complex of bacterial 30S subunit with initiator tRNA, mRNA, and IFs 1-3, representing different steps along the initiation pathway. IF1 provides key anchoring points for IF2 and IF3, thereby enhancing their activities. IF2 positions a domain in an extended conformation appropriate for capturing the formylmethionyl moiety charged on tRNA. IF3 and tRNA undergo large conformational changes to facilitate the accommodation of the formylmethionyl-tRNA (fMet-tRNA(fMet)) into the P site for start codon recognition.

PubMed: 27662086
DOI: 10.1016/j.cell.2016.08.074

Experimental method

ELECTRON MICROSCOPY (4.9 Å)