5LKH

Cryo-EM structure of the Tc toxin TcdA1 in its pore state (obtained by flexible fitting)

5LKH の概要

• エントリーDOI: 10.2210/pdb5lkh/pdb
• 関連するPDBエントリー: 5LKI
• EMDBエントリー: 4068
• 分子名称: TcdA1 (1 entity in total)
• 機能のキーワード: nanodisc, toxin, injection, pore-forming
• 由来する生物種: Photorhabdus luminescens
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 1416147.03

構造登録者

Gatsogiannis, C.,Merino, F.,Prumbaum, D.,Roderer, D.,Leidreiter, F.,Meusch, D.,Raunser, S. (登録日: 2016-07-22, 公開日: 2016-08-31, 最終更新日: 2024-05-08)

主引用文献

Gatsogiannis, C.,Merino, F.,Prumbaum, D.,Roderer, D.,Leidreiter, F.,Meusch, D.,Raunser, S.
Membrane insertion of a Tc toxin in near-atomic detail.
Nat.Struct.Mol.Biol., 23:884-890, 2016

PubMed Abstract: Tc toxins from pathogenic bacteria use a special syringe-like mechanism to perforate the host cell membrane and inject a deadly enzyme into the host cytosol. The molecular mechanism of this unusual injection system is poorly understood. Using electron cryomicroscopy, we determined the structure of TcdA1 from Photorhabdus luminescens embedded in lipid nanodiscs. In our structure, compared with the previous structure of TcdA1 in the prepore state, the transmembrane helices rearrange in the membrane and open the initially closed pore. However, the helices do not span the complete membrane; instead, the loops connecting the helices form the rim of the funnel. Lipid head groups reach into the space between the loops and consequently stabilize the pore conformation. The linker domain is folded and packed into a pocket formed by the other domains of the toxin, thereby considerably contributing to stabilization of the pore state.

PubMed: 27571177
DOI: 10.1038/nsmb.3281

実験手法

ELECTRON MICROSCOPY (3.46 Å)