5LK4
Structure of the Red Fluorescent Protein mScarlet at pH 7.8
Summary for 5LK4
| Entry DOI | 10.2210/pdb5lk4/pdb |
| Descriptor | mScarlet, TETRAETHYLENE GLYCOL, PHOSPHATE ION, ... (6 entities in total) |
| Functional Keywords | beta barrel, red fluorescent protein, fluorescent protein |
| Biological source | Discosoma sp. |
| Total number of polymer chains | 1 |
| Total formula weight | 25893.09 |
| Authors | Aumonier, S.,Gotthard, G.,Royant, A. (deposition date: 2016-07-20, release date: 2016-12-07, Last modification date: 2024-01-31) |
| Primary citation | Bindels, D.S.,Haarbosch, L.,van Weeren, L.,Postma, M.,Wiese, K.E.,Mastop, M.,Aumonier, S.,Gotthard, G.,Royant, A.,Hink, M.A.,Gadella, T.W. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat. Methods, 14:53-56, 2017 Cited by PubMed Abstract: We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet starting with a consensus synthetic template and using improved spectroscopic screening techniques; mScarlet's crystal structure reveals a planar and rigidified chromophore. mScarlet outperforms existing red fluorescent proteins as a fusion tag, and it is especially useful as a Förster resonance energy transfer (FRET) acceptor in ratiometric imaging. PubMed: 27869816DOI: 10.1038/nmeth.4074 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.47 Å) |
Structure validation
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