5LIV

Crystal structure of myxobacterial CYP260A1

5LIV の概要

• エントリーDOI: 10.2210/pdb5liv/pdb
• 分子名称: Cytochrome P450 CYP260A1,Cytochrome P450 CYP260A1, PROTOPORPHYRIN IX CONTAINING FE, GLYCEROL, ... (7 entities in total)
• 機能のキーワード: 1-alpha-hydroxylase, steroid, sorangium cellulosum, redox pool, oxidoreductase
• 由来する生物種: Sorangium cellulosum So ce56; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 205042.82

構造登録者

Carius, Y.,Khatri, Y.,Bernhardt, R.,Lancaster, C.R.D. (登録日: 2016-07-15, 公開日: 2016-11-23, 最終更新日: 2024-01-10)

主引用文献

Khatri, Y.,Carius, Y.,Ringle, M.,Lancaster, C.R.,Bernhardt, R.
Structural characterization of CYP260A1 from Sorangium cellulosum to investigate the 1 alpha-hydroxylation of a mineralocorticoid.
FEBS Lett., 590:4638-4648, 2016

PubMed Abstract: In this study, we report the crystal structure of the cytochrome P450 CYP260A1 (PDB 5LIV) from the myxobacterium Sorangium cellulosum So ce56. In addition, we investigated the hydroxylation of 11-deoxycorticosterone by CYP260A1 by reconstituting the enzyme with the surrogate redox partners adrenodoxin and adrenodoxin reductase. The major product of this steroid conversion was identified as 1α-hydroxy-11-deoxycorticosterone, a novel Δ4 C-21 steroidal derivative. Furthermore, we docked the substrate into the crystal structure and replaced Ser326, the residue responsible for substrate orientation, with asparagine and observed that the mutant S326N displayed higher activity and selectivity for the formation of 1α-hydroxy-11-deoxycorticosterone compared to the wild-type CYP260A1. Thus, our findings highlight the usefulness of the obtained crystal structure of CYP260A1 in identifying biotechnologically more efficient reactions.

PubMed: 27878817
DOI: 10.1002/1873-3468.12479

実験手法

X-RAY DIFFRACTION (2.67 Å)