5LEG

Structure of the bacterial sex F pilus (pED208)

これはPDB形式変換不可エントリーです。

5LEG の概要

• エントリーDOI: 10.2210/pdb5leg/pdb
• EMDBエントリー: 4042
• 分子名称: Pilin, 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE (2 entities in total)
• 機能のキーワード: f-pilus conjugation type iv secretion system phospholipid, protein fibril
• 由来する生物種: Salmonella enterica subsp. enterica serovar Typhi
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein: P12060
• タンパク質・核酸の鎖数: 80
• 化学式量合計: 594242.75

構造登録者

Costa, T.R.D.,Ilangovan, I.,Ukleja, M.,Redzej, A.,Santini, J.M.,Smith, T.K.,Egelman, E.H.,Waksman, G. (登録日: 2016-06-29, 公開日: 2016-09-28, 最終更新日: 2024-05-15)

主引用文献

Costa, T.R.,Ilangovan, A.,Ukleja, M.,Redzej, A.,Santini, J.M.,Smith, T.K.,Egelman, E.H.,Waksman, G.
Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex.
Cell, 166:1436-1444.e10, 2016

PubMed Abstract: Conjugative pili are widespread bacterial appendages that play important roles in horizontal gene transfer, in spread of antibiotic resistance genes, and as sites of phage attachment. Among conjugative pili, the F "sex" pilus encoded by the F plasmid is the best functionally characterized, and it is also historically the most important, as the discovery of F-plasmid-mediated conjugation ushered in the era of molecular biology and genetics. Yet, its structure is unknown. Here, we present atomic models of two F family pili, the F and pED208 pili, generated from cryoelectron microscopy reconstructions at 5.0 and 3.6 Å resolution, respectively. These structures reveal that conjugative pili are assemblies of stoichiometric protein-phospholipid units. We further demonstrate that each pilus type binds preferentially to particular phospholipids. These structures provide the molecular basis for F pilus assembly and also shed light on the remarkable properties of conjugative pili in bacterial secretion and phage infection.

PubMed: 27610568
DOI: 10.1016/j.cell.2016.08.025

実験手法

ELECTRON MICROSCOPY (3.6 Å)