5LCW

Cryo-EM structure of the Anaphase-promoting complex/Cyclosome, in complex with the Mitotic checkpoint complex (APC/C-MCC) at 4.2 angstrom resolution

5LCW の概要

• エントリーDOI: 10.2210/pdb5lcw/pdb
• EMDBエントリー: 4037
• 分子名称: Anaphase-promoting complex subunit 1, Anaphase-promoting complex subunit 10, Anaphase-promoting complex subunit 13, ... (18 entities in total)
• 機能のキーワード: complex, ubiquitin, e3 ligase, ubiquitin ligase, cullin, ring, cell cycle, mitosis, spindle checkpoint, degron
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 1331085.90

構造登録者

Alfieri, C.,Chang, L.,Zhang, Z.,Yang, J.,Maslen, S.,Skehel, M.,Barford, D. (登録日: 2016-06-22, 公開日: 2016-08-10, 最終更新日: 2024-10-09)

主引用文献

Alfieri, C.,Chang, L.,Zhang, Z.,Yang, J.,Maslen, S.,Skehel, M.,Barford, D.
Molecular basis of APC/C regulation by the spindle assembly checkpoint.
Nature, 536:431-436, 2016

PubMed Abstract: In the dividing eukaryotic cell, the spindle assembly checkpoint (SAC) ensures that each daughter cell inherits an identical set of chromosomes. The SAC coordinates the correct attachment of sister chromatid kinetochores to the mitotic spindle with activation of the anaphase-promoting complex (APC/C), the E3 ubiquitin ligase responsible for initiating chromosome separation. In response to unattached kinetochores, the SAC generates the mitotic checkpoint complex (MCC), which inhibits the APC/C and delays chromosome segregation. By cryo-electron microscopy, here we determine the near-atomic resolution structure of a human APC/C–MCC complex (APC/C(MCC)). Degron-like sequences of the MCC subunit BubR1 block degron recognition sites on Cdc20, the APC/C coactivator subunit responsible for substrate interactions. BubR1 also obstructs binding of the initiating E2 enzyme UbcH10 to repress APC/C ubiquitination activity. Conformational variability of the complex enables UbcH10 association, and structural analysis shows how the Cdc20 subunit intrinsic to the MCC (Cdc20(MCC)) is ubiquitinated, a process that results in APC/C reactivation when the SAC is silenced.

PubMed: 27509861
DOI: 10.1038/nature19083

実験手法

ELECTRON MICROSCOPY (4.2 Å)