5L8M

Wild-type PAS-GAF fragment from Deinococcus radiodurans Bphp collected at LCLS

Summary for 5L8M

• Entry DOI: 10.2210/pdb5l8m/pdb
• Related: 5K5B
• Descriptor: Bacteriophytochrome, 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid, ACETATE ION, ... (4 entities in total)
• Functional Keywords: kinase, photosensor, transferase, phytochrome
• Biological source: Deinococcus radiodurans
• Total number of polymer chains: 1
• Total formula weight: 37874.17

Authors

Claesson, E.,Takala, H.,Edlund, P.,Henry, L.,Dods, R.,Schmidt, M.,Westenhoff, S. (deposition date: 2016-06-08, release date: 2016-10-26, Last modification date: 2024-01-10)

Primary citation

Edlund, P.,Takala, H.,Claesson, E.,Henry, L.,Dods, R.,Lehtivuori, H.,Panman, M.,Pande, K.,White, T.,Nakane, T.,Berntsson, O.,Gustavsson, E.,Bath, P.,Modi, V.,Roy-Chowdhury, S.,Zook, J.,Berntsen, P.,Pandey, S.,Poudyal, I.,Tenboer, J.,Kupitz, C.,Barty, A.,Fromme, P.,Koralek, J.D.,Tanaka, T.,Spence, J.,Liang, M.,Hunter, M.S.,Boutet, S.,Nango, E.,Moffat, K.,Groenhof, G.,Ihalainen, J.,Stojkovic, E.A.,Schmidt, M.,Westenhoff, S.
The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography.
Sci Rep, 6:35279-35279, 2016

PubMed Abstract: Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophore-binding domains of the Deinococcus radiodurans phytochrome at 2.1 Å resolution. The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser. We find overall good agreement compared to a crystal structure at 1.35 Å resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX.

PubMed: 27756898
DOI: 10.1038/srep35279

Experimental method

X-RAY DIFFRACTION (2.1 Å)