5L80

Structure of Exuperantia EXO-like and SAM-like domains

5L80 の概要

• エントリーDOI: 10.2210/pdb5l80/pdb
• 関連するPDBエントリー: 5L7Z
• 分子名称: Maternal protein exuperantia,Maternal protein exuperantia (2 entities in total)
• 機能のキーワード: exonuclease, rna, pseudo nuclease, sam domain, rna binding protein
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 79401.34

構造登録者

Lazzaretti, D.,Veith, K.,Bono, F. (登録日: 2016-06-05, 公開日: 2016-07-06, 最終更新日: 2024-01-10)

主引用文献

Lazzaretti, D.,Veith, K.,Kramer, K.,Basquin, C.,Urlaub, H.,Irion, U.,Bono, F.
The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease.
Nat.Struct.Mol.Biol., 23:705-713, 2016

PubMed Abstract: Anterior patterning in Drosophila is mediated by the localization of bicoid (bcd) mRNA at the anterior pole of the oocyte. Exuperantia (Exu) is a putative exonuclease (EXO) associated with bcd and required for its localization. We present the crystal structure of Exu, which reveals a dimeric assembly with each monomer consisting of a 3'-5' EXO-like domain and a sterile alpha motif (SAM)-like domain. The catalytic site is degenerate and inactive. Instead, the EXO-like domain mediates dimerization and RNA binding. We show that Exu binds RNA directly in vitro, that the SAM-like domain is required for RNA binding activity and that Exu binds a structured element present in the bcd 3' untranslated region with high affinity. Through structure-guided mutagenesis, we show that Exu dimerization is essential for bcd localization. Our data demonstrate that Exu is a noncanonical RNA-binding protein with EXO-SAM-like domain architecture that interacts with its target RNA as a homodimer.

PubMed: 27376588
DOI: 10.1038/nsmb.3254

実験手法

X-RAY DIFFRACTION (2.8 Å)